Real Time PCR
12 STD panel genotyping test

Real Time PCR Test for 12 different STDs

About MF STD12
MF STD12 is a real time PCR detection kit for 12 different STDs. The kit utilizes an exogenous internal positive control to be simultaneously amplified with the pathogen target to rule out PCR inhibition and decrease false negatives. In addition, the kit incorporates uracil DNA glycosylase (UDG) to minimize carry over contamination and false positives. The MF STD12 kit provides results in just under 3 hours for same day turnaround times and works with the following three specimen types: swab specimen, urine specimen, or liquid based cytology specimen.
List of STDs detected
Oligo mixture Oligo mixture (Tube A) Oligo mixture (Tube B) Oligo mixture (Tube C)
Pathogens Chlamydia trachomatis
Neisseria gonorrhoeae
Ureaplasma parvum
Mycoplasma genitalium
Ureaplasma urealyticum
Mycoplasma hominis
Candida albicans
Gardnerella vaginalis
Treponema pallidum
Trichomonas vaginalis
Compatible specimen types
Swab specimen Urine specimen Liquid based cytology specimen
Urethral, vaginal and cervical swab Standard urine specimen ThinPrep® and SurePath™
Mediforum MF STD12 Results
Figure 1
Multiple amplification of positive control mixtures (A, B and C) containing exogenous internal positive controls in an assay by the MF STD12 real time PCR detection kit
Mediforum MF STD12 Results
Figure 2
Sensitive detection of 12 targets in an assay by the MF STD12 real time PCR detection kit. 10-fold serial dilutions of positive control mixtures (106, 105, 104, 103, and 102 copies/reaction) were analyzed using target specific primer and probe sets.
Equipment Used
We highly recommend using the CFX96 Real Time PCR Detection System from Bio-Rad for their open systems and ease of use.
Mediforum MF STD12 Test Equipment
Product Details
Product Name SKU Test Quantity per Kit Certifications Qualifications
MF STD12 Kit MF2003 144 individual tests CE Mark K-GMP, ISO 13485